ตัวกลางเพาะเชื้อ Ashdown's medium เป็นตัวกลางที่มียาปฏิชีวนะเจนตามัยซิน (gentamicin) ซึ่งจำเป็นในการเพาะเชื้อจากส่วนที่ไม่ปราศจากเชื้อ (non-sterile sites) ตัวกลางเพาะเชื้อ Burkholderia cepacia medium อาจเป็นตัวเลือกที่ใช้เพาะเชื้อได้ในบริเวณที่ไม่ใช่พื้นที่โรคเมลิออยด์เป็นโรคประจำถิ่นหากไม่มี Ashdown's medium ให้ใช้ ตัวกลางเพาะเชื้อชนิดใหม่ซึ่งพัฒนามาจาก Ashdown's medium ชื่อว่า Francis medium อาจช่วยในการแยกระหว่างเชื้อ B. pseudomallei กับ B. cepacia และอาจช่วยในการวินิจฉัยโรคเมลิออยด์ได้ในระยะแรก[53] แต่ก็ยังไม่มีการยอมรับกันทางคลินิกอย่างกว้างขวาง ชุดตรวจเพื่อยืนยันเชื้อแบคทีเรียสำเร็จรูปหลายตัวอาจไม่สามารถระบุเชื้อ B. pseudomallei ได้อย่างถูกต้อง
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