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De Mos grew up in Hook of Holland, and taught in a primary school in the Spoorwijk in The Hague. He became policy officer of the PVV MP Martin Bosma in 2007. On 1 September 2009, De Mos took over the seat of Barry Madlener, who had been elected into the European Parliament. In the House of Representatives, he focused on matters of environmental policy, climate change, waterways, day care and taxicab policy. De Mos was not selected to contest in the 2012 general election by party leader Geert Wilders. On 11 March 2010, he became a member of the municipal council of The Hague, initially for the Party for Freedom, later as an independent. He contested in the 2014 municipal elections under Groep De Mos/Ouderenpartij, and won three seats in the council.
His party grew to eight seats in the 2018 municipal election, becoming the largest party. He subsequently became the city's First Deputy Mayor and Alderman for Economic Affairs, Sport and Public Space.
He is in favour of the reintroduction of vocational schools and is highly sceptical toward man-made global warming.