Diagram of a typical organ bath preparation. An excised piece of smooth muscle tissue is held in an oxygenated solution in a chamber. The tissue is attached to a lever, which transmits its contraction to a myograph, thus recording the physiological response. Drugs under investigation can be administered directly to the chamber
An organ chamber, organ bath (or, colloquially, gut bath) is a chamber in which isolated organs or tissues can be administered with drugs, or stimulated electrically, in order to measure their function. The tissue in the organ bath is typically oxygenated with carbogen and kept in a solution such as Tyrode's solution or lactated Ringer's solution.
The use of organ bath preparations for the measurement of physiological tissue responses to drug concentrations allows the generation of dose response curves. This in turn allows the quantification of a drug's pharmacological profile in the tissue in question, such as the calculation of the drug's EC50, IC50, and Hill coefficient.
Examples of important contributions made using this technique include:
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