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A microaerophile is a microorganism that requires oxygen to survive, but requires environments containing lower levels of oxygen than are present in the atmosphere (i.e. <21% O2; typically 2–10% O2).[1][2] Many microaerophiles are also capnophiles, requiring an elevated concentration of carbon dioxide (e.g. 10% CO2 in the case of Campylobacter species).[3]
Microaerophiles can be cultivated in candle jars. Candle jars are containers into which a lit candle is introduced before sealing the container's airtight lid. The candle's flame burns until extinguished by oxygen deprivation, creating a carbon dioxide-rich, oxygen-poor atmosphere.[4] Other methods of creating a microaerobic environment include using a gas-generating pack and gas exchange.[3]
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