King is known for three major accomplishments: demonstrating that humans and chimpanzees are 99% genetically identical; identifying breast cancer genes; and applying genomic sequencing to identify victims of human rights abuses. In 1984, in Argentina, she began working with Abuelas de Plaza de Mayo (Grandmothers of Plaza de Mayo) in identifying children who had been stolen from their families and adopted illegally under the military dictatorship during the Dirty War (1976-1983). In 2002, Discover magazine recognized King as one of the 50 most important women in science.
Mary-Claire King was born on February 27, 1946, to Harvey and Clarice King of Wilmette, Illinois, near Chicago. Her father worked for Standard Oil of Indiana.
When King was 15 years old, her childhood best friend died of cancer. King became interested in science in the hope of learning enough to prevent and treat such illnesses.
King was accepted into the graduate program at the University of California, Berkeley, and soon became politically active. She helped to organize protests against U.S. involvement in the Vietnam War in 1970. She says: "The single most effective thing we did was on the day after the US invaded Cambodia, we got out our suit jackets and shirtwaist dresses – not clothes that any of us had worn since coming to Berkeley – and went to the synagogues and churches and by the end of Sunday we had 30,000 letters opposing the action."
She dropped out of university briefly after the National Guard was sent in against student protestors.
She spent a year doing consumer advocacy work for Ralph Nader, investigating pesticide use and its effects on farm workers.
After her return to Berkeley, advisor Allan Wilson persuaded her to switch from mathematics to genetics. King had been introduced to genetics by professor Curt Stern, in the last class he taught before his retirement.
In her doctoral work at Berkeley, King demonstrated through comparative protein analysis that chimpanzees and humans were 99% genetically identical. King's work supported Allan Wilson's view that chimpanzees and humans diverged only five million years ago, and King and Wilson suggested that gene regulation was likely responsible for the significant differences between the species. King completed her thesis in 1972, and received her doctorate in genetics from the University of California, Berkeley in 1973.
Next King went to Santiago, Chile to teach at the Universidad de Chile as part of a University of California-University of Chile exchange program. Her time there was cut short when the Chilean government of Salvador Allende was overthrown in a CIA-backed military coup on September 11, 1973. King and her husband Robert Colwell returned to Berkeley in late December.
She later learned that a number of her colleagues and students had either disappeared or been killed.
King accepted a postdoctoral position at the University of California, San Francisco (UCSF), to work with Nicholas L. Petrakis. As of January 1, 1974, King began to work on the problem of why breast cancer tends to appear in families.
King's younger brother Paul King, a mathematician and business consultant, was the CEO of Vanalco in Vancouver, Washington.
King married ecologist Robert K. Colwell in 1973 . They have one child, Emily King Colwell, born in 1975. Emily studied the evolution of languages at Brown University with a B.A. in linguistics. King and Colwell divorced in 1983.
King accepted a faculty appointment at the University of California, Berkeley, as professor of genetics and epidemiology in 1976. She remained at UC Berkeley until 1995, when she accepted an appointment as the American Cancer Society Professor at the University of Washington.
From 1974 to 1990, King carried out years of painstaking research, seeking a genetic marker, an identifiable gene that tended to accompany the presence of breast cancer in families. For much of that time, the predominant theory was that cancer was viral. Most scientists disregarded or attacked her ideas. The idea that genetic patterns could be linked to the incidence of complex diseases was considered an unlikely long shot. Genetics had been recognized as significant in diseases with a simple genetic tie, such as Huntington's disease, cystic fibrosis, and sickle-cell anemia, but researchers were skeptical about the usefulness of genetics in studying more common and complex diseases involving both multiple genetic factors and environmental influences.
King sometimes worried that she was going down a blind alley in trying to study the interplay of genetics with a complex human disease.
By 1990, King and her team had examined and rejected 172 possible markers using a technique called family linkage analysis. Then one of the team members suggested that they reorganize their data by age of onset, focusing on families in which members had developed cancer at a relatively young age. The idea was that early cases might be more likely to reflect a genetic component, in contrast to sporadic mutations that might occur at any age, or even accrue over time.
King's group were able to demonstrate that a single gene on chromosome 17 could be linked to many breast and ovarian cancers. As many as 5-10% of all cases of breast cancer may be hereditary. In 1991 King officially named the gene BRCA1.
Her discovery paved the way for identification of the gene sequence. In September 1994, Myriad Genetics published a paper on the positional cloning of the sequence after a highly publicized "race" by groups of scientists. In December 1994, King and her collaborators published results based on a second cohort of families. A second gene, BRCA2, was also found. These two genes, BRCA 1 and BRCA 2 work together to clean up cells in the body that have been harmed by things such as tobacco or just help clean the cells because they have aged. When these genes do not perform these functions, it causes cells to grow and divide quickly, leading to some types of cancers. Both genes worked to suppress the development of cancer tumors, but certain types of genetic mutations could prevent them from doing so.
In 1996, with support from the Breast Cancer Research Foundation (BCRF) Mary-Claire King and social worker Joan Marks began the New York Breast Cancer Study, which definitively determined that incidence of breast and ovarian cancer was linked to inherited mutations of the genes BRCA1 and BRCA2. The researchers studied women of Ashkenazi Jewish ancestry in New York, a group that was known to have a very high incidence of breast cancer (up to an 80% risk by age 70 compared to 12% in the general population). She has also studied the incidence of breast cancer in Palestinian women.
The discovery of the "breast cancer gene" revolutionized the study of numerous other common diseases. The technique King developed to identify BRCA1 has since proven valuable in the study of many other illnesses and conditions. King's contributions have made it possible for people to be informed of genetic information that then can aid them in making choices best for themselves and for their future.
Mary-Clarie King also worked on a project studying the mutations of genes linked to breast cancer inheritance in Nigerian women between March 1998 and 2014. King's team decided to do this research on the grounds that more people die in Nigeria from triple negative breast cancer that is diagnosed at a later stage than other, more educated regions of the world, such as Europe or America. At the finish of this study, King's team was still unsure of the reason for such high levels of Triple-negative breast cancer, since many of the people diagnosed were not showing mutations in the BRCA 1 gene. Her study supported the idea that genomic sequencing could be useful as a tool to help detect gene mutations early and be proactive in letting those who have high risks for breast cancer know ahead of time.
King continues to work with scientists Karen Avraham at Tel Aviv University in Israel and Moien Kanaan at Bethlehem University in Palestine, modeling international scientific cooperation in conjunction with conducting scientific research. Hereditary deafness is common among some Palestinian and Israeli communities, providing good study populations to understand the genetics of this condition.
We understand that working together transcends politics. It’s possible to do beautiful useful science together, even between countries that are in conflict.
King in recent years has developed a deep interest in studying the genetic factors influencing schizophrenia. In collaboration with other scientists including Judith L. Rapoport, Jonathan Sebat, and Deborah L. Levy, she has discovered evidence that suggests that schizophrenia may be linked to the presence of genetic mutations called copy number variations (CNVs) in pathways involved in neural development. A percentage of such variations may occur spontaneously as a result of copying errors in cells.
King is well known for her work on the forensic determination of individual identity, in particular the use of mitochondrial DNA from teeth.
King was invited to participate in DNA investigations of the first analysis of Romanov remains exhumed in 1991 in Ekaterinburg, Russia.
Human rights work
King first applied her genetics skills to human rights work in 1984, when she and her lab began working with Abuelas de Plaza de Mayo (Grandmothers of Plaza de Mayo) in Argentina. She used dental genetics to identify missing persons, ultimately identifying 59 children and helping return them to their biological families. Most had been born to women in prison who had been persecuted as political dissidents and were later "disappeared" by the Argentine military dictatorship during the eight-year "Dirty War" from 1976-1983. These children were often illegally "adopted" by military families without their mother's or other family consent.
Beginning in 1977 Las Abuelas ("the grandmothers") had gathered to protest the disappearance of their grandchildren and seek their return. Every Thursday, they marched to the central plaza in Buenos Aires ("Plaza de Mayo") to demand the return of their grandchildren, and they began gathering data trying to identify the many missing children (estimated to be 400-500).
By the time King joined the project, the dictatorship had been replaced by a democratic government, but it required proof of kinship to remove children from families and return them to biological families. King's technique, using mitochondrial DNA and human leukocyte antigen serotyping genetic markers from dental samples, proved invaluable. The Supreme Court of Argentina in 1984 determined that King's test had positively identified the relationship of Paula Logares to her family, establishing the precedent for the ultimate reunification of dozens of families with their stolen children.
Since 1984, this technique has become a major method for genetic identification of the deceased as well as the living. In 1993 King used the technique to identify the remains of individuals massacred in the village of El Mozote, El Salvador. More than 750 adults and children were massacred and buried in mass graves by US-trained Salvadoran soldiers of the Atlacatl Battalion.
King has worked with numerous human rights organizations, such as Physicians for Human Rights and Amnesty International, to identify missing people in countries including Argentina, Chile, El Salvador, Guatemala, Haiti, Honduras, Mexico, Rwanda, the Balkans (Croatia and Serbia), and the Philippines. King's lab has also provided DNA identification for the U.S. Army, the United Nations, and the U.N.'s war crimes tribunals.
King has been supportive of women and ethnic and sexual minorities in science. She has been highly critical of genetic patenting, a practice which was ruled against by the U.S. Supreme Court in 2013, on the grounds that "genes are natural products and cannot be patented".
Decoding Annie Parker
The movie Decoding Annie Parker (2013) depicts King's quest to discover how genetics could be a cause of beast cancer. In the movie, Helen Hunt portrays Mary-Claire King and Annie Parker is played by Samantha Morton. The movie focuses on Annie and her trials to get King's help. Both Annie's mother and sister were diagnosed with breast cancer and Annie feels that the same could be in her future. Annie gets cancer a total of three times, fighting it off each time. A reviewer described the movie's version of King as "a taut, unsmiling crusader". The film was made without King's knowledge or consent, and she learned of it only by accident. Much of the story was fictionalized. Annie Parker had not written to her, and they met only after the film was released.
Awards, prizes, and honors
Dr. King has won numerous awards, prizes, and honors for her scientific and humanitarian work, including:.
^Hall, J.; Lee, M.; Newman, B.; Morrow, J.; Anderson, L.; Huey, B.; King, M. (1990). "Linkage of early-onset familial breast cancer to chromosome 17q21". Science. 250 (4988): 1684–1689. doi:10.1126/science.2270482. PMID2270482.
^ abMiki, Y; Swensen, J; Shattuck-Eidens, D; Futreal, P.; Harshman, K; Tavtigian, S; Liu, Q; Cochran, C; Bennett, L.; Ding, W; et, al. (7 October 1994). "A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1". Science. 266 (5182): 66–71. doi:10.1126/science.7545954. PMID7545954.
^Friedman, Lori S.; Ostermeyer, Elizabeth A.; Szabo, Csilla I.; Dowd, Patrick; Lynch, Eric D.; Rowell, Sarah E.; King, Mary-Claire (December 1994). "Confirmation of BRCA1 by analysis of germline mutations linked to breast and ovarian cancer in ten families". Nature Genetics. 8 (4): 399–404. doi:10.1038/ng1294-399. PMID7894493.
^Lynch, E. D. (14 November 1997). "Nonsyndromic Deafness DFNA1 Associated with Mutation of a Human Homolog of the Drosophila Gene diaphanous". Science. 278 (5341): 1315–1318. doi:10.1126/science.278.5341.1315.
^Walsh, T.; McClellan, J. M.; McCarthy, S. E.; Addington, A. M.; Pierce, S. B.; Cooper, G. M.; Nord, A. S.; Kusenda, M.; Malhotra, D.; Bhandari, A.; Stray, S. M.; Rippey, C. F.; Roccanova, P.; Makarov, V.; Lakshmi, B.; Findling, R. L.; Sikich, L.; Stromberg, T.; Merriman, B.; Gogtay, N.; Butler, P.; Eckstrand, K.; Noory, L.; Gochman, P.; Long, R.; Chen, Z.; Davis, S.; Baker, C.; Eichler, E. E.; Meltzer, P. S.; Nelson, S. F.; Singleton, A. B.; Lee, M. K.; Rapoport, J. L.; King, M.-C.; Sebat, J. (25 April 2008). "Rare Structural Variants Disrupt Multiple Genes in Neurodevelopmental Pathways in Schizophrenia". Science. 320 (5875): 539–543. doi:10.1126/science.1155174.
^Owens, Kelly N; Harvey-Blankenship, Michelle; King, Mary-Claire (February 2002). "Genomic sequencing in the service of human rights". International Journal of Epidemiology. 31 (1): 53–58. doi:10.1093/ije/31.1.53.
^King, MC (1991). "An application of DNA sequencing to a human rights problem". Molecular Genetic Medicine. 1: 117–31. PMID1842570.