Acid-citrate-dextrose or acid-citrate-dextrose solution, also known as anticoagulant-citrate-dextrose or anticoagulant-citrate-dextrose solution (and often styled without the hyphens between the coordinate terms, thus acid citrate dextrose or ACD) is any solution of citric acid, sodium citrate, and dextrose in water. It is mainly used as an anticoagulant (in yellow top tubes) to preserve blood specimens required for tissue typing. It is also used during procedures such as plasmapheresis instead of heparin.
Two solutions (A and B) are defined by the United States Pharmacopeia. They have the following properties:
|Total Citrate (as citric acid, anhydrous (C6H8O7))||20.59 to 22.75g|
|Dextrose (C6H12O6*H2O)||23.28g to 25.73g|
|Sodium (Na)||4.90g to 5.42g|
|Total Citrate (as citric acid, anhydrous (C6H8O7))||12.37 to 13.67g|
|Dextrose (C6H12O6*H2O)||13.96 to 15.44g|
|Sodium (Na)||2.94 to 3.25g|
To make use:
|Citric acid, anhydrous (C6H8O7)||7.3g||4.4|
|Sodium citrate, dihydrate||22.0g||13.2|
|Dextrose, monohydrate (C6H12O6*H2O)||24.5g||14.7|
|Water for injection to make||1000 mL||1000 mL|
Dissolve the ingredients and mix. Filter until clear.
United States Pharmacopeia 26, 2002, pp 158.