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Acid-citrate-dextrose or acid-citrate-dextrose solution, also known as anticoagulant-citrate-dextrose or anticoagulant-citrate-dextrose solution (and often styled without the hyphens between the coordinate terms, thus acid citrate dextrose or ACD) is any solution of citric acid, sodium citrate, and dextrose in water. It is mainly used as an anticoagulant (in yellow top tubes)[1] to preserve blood specimens required for tissue typing. It is also used during procedures such as plasmapheresis instead of heparin.

Two solutions (A and B) are defined by the United States Pharmacopeia. They have the following properties:

Solution A (per 1000 mL)
Total Citrate (as citric acid, anhydrous (C6H8O7)) 20.59 to 22.75g
Dextrose (C6H12O6*H2O) 23.28g to 25.73g
Sodium (Na) 4.90g to 5.42g
Solution B (per 1000 mL)
Total Citrate (as citric acid, anhydrous (C6H8O7)) 12.37 to 13.67g
Dextrose (C6H12O6*H2O) 13.96 to 15.44g
Sodium (Na) 2.94 to 3.25g

To make use:

Citric acid, anhydrous (C6H8O7) 7.3g 4.4
Sodium citrate, dihydrate 22.0g 13.2
Dextrose, monohydrate (C6H12O6*H2O) 24.5g 14.7
Water for injection to make 1000 mL 1000 mL

Dissolve the ingredients and mix. Filter until clear.


United States Pharmacopeia 26, 2002, pp 158.

  1. ^ "ORDER OF DRAW FOR MULTIPLE TUBE COLLECTIONS" (PDF). Michigan Medicine Laboratories. 2019-09-15. Retrieved 2020-03-27.

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