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Prolactin synthesized and secreted by human peripheral blood mononuclear cells: an autocrine growth factor for lymphoproliferation.

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US National Library of Medicine
National Institutes of Health Proc Natl Acad Sci U S A. 1992 Aug 15; 89(16): 7713–7716. doi: 10.1073/pnas.89.16.7713 PMCID: PMC49781 PMID: 1502189

Prolactin synthesized and secreted by human peripheral blood mononuclear cells: an autocrine growth factor for lymphoproliferation.

P Sabharwal, R Glaser, W Lafuse, S Varma, Q Liu, S Arkins, R Kooijman, L Kutz, K W Kelley, and W B Malarkey Author information Copyright and License information Disclaimer Department of Medicine, Ohio State University Medical Center, Columbus 43210. Copyright notice This article has been cited by other articles in PMC.


Prolactin has been shown to have an immunoregulatory role in the rodent immune response. A prolactin-like molecule has also been found in mouse splenocytes and a human B-lymphoblastoid cell line. We have evaluated whether human peripheral blood mononuclear cells (PBMCs) synthesize and/or secrete prolactin. We used the polymerase chain reaction (PCR) to generate a 276-base-pair prolactin product from human PBMCs, and Southern blot analysis confirmed that it was related to prolactin. Western blotting using a polyclonal antibody to prolactin indicated that cell extracts prepared from human PBMCs contained a high molecular mass (60-kDa) immunoreactive prolactin. To determine whether this PBMC prolactin was being secreted, we developed a highly sensitive and specific hormonal enzyme-linked immunoplaque assay. With this assay, we were able to detect human prolactin secretion from concanavalin A (Con A)- or phytohemagglutinin-stimulated PBMCs but not from unstimulated PBMCs. We next sought to determine whether this secreted prolactin could function as an autocrine growth factor in lymphoproliferation. We observed that anti-human prolactin antiserum significantly inhibited human PBMC proliferation in response to Con A or phytohemagglutinin. We conclude that a prolactin-like molecule is synthesized and secreted by human PBMCs and that it functions in an autocrine manner as a growth factor for lymphoproliferation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Nagy E, Berczi I. Immunodeficiency in hypophysectomized rats. Acta Endocrinol (Copenh) 1978 Nov;89(3):530–537. [PubMed] [Google Scholar]
  • Bernton EW, Meltzer MS, Holaday JW. Suppression of macrophage activation and T-lymphocyte function in hypoprolactinemic mice. Science. 1988 Jan 22;239(4838):401–404. [PubMed] [Google Scholar]
  • Montgomery DW, Zukoski CF, Shah GN, Buckley AR, Pacholczyk T, Russell DH. Concanavalin A-stimulated murine splenocytes produce a factor with prolactin-like bioactivity and immunoreactivity. Biochem Biophys Res Commun. 1987 Jun 15;145(2):692–698. [PubMed] [Google Scholar]
  • DiMattia GE, Gellersen B, Bohnet HG, Friesen HG. A human B-lymphoblastoid cell line produces prolactin. Endocrinology. 1988 Jun;122(6):2508–2517. [PubMed] [Google Scholar]
  • Baglia LA, Cruz D, Shaw JE. An Epstein-Barr virus-negative Burkitt lymphoma cell line (sfRamos) secretes a prolactin-like protein during continuous growth in serum-free medium. Endocrinology. 1991 May;128(5):2266–2272. [PubMed] [Google Scholar]
  • Clevenger CV, Russell DH, Appasamy PM, Prystowsky MB. Regulation of interleukin 2-driven T-lymphocyte proliferation by prolactin. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6460–6464. [PMC free article] [PubMed] [Google Scholar]
  • Hartmann DP, Holaday JW, Bernton EW. Inhibition of lymphocyte proliferation by antibodies to prolactin. FASEB J. 1989 Aug;3(10):2194–2202. [PubMed] [Google Scholar]
  • Clevenger CV, Altmann SW, Prystowsky MB. Requirement of nuclear prolactin for interleukin-2--stimulated proliferation of T lymphocytes. Science. 1991 Jul 5;253(5015):77–79. [PubMed] [Google Scholar]
  • Truong AT, Duez C, Belayew A, Renard A, Pictet R, Bell GI, Martial JA. Isolation and characterization of the human prolactin gene. EMBO J. 1984 Feb;3(2):429–437. [PMC free article] [PubMed] [Google Scholar]
  • Montgomery DW, LeFevre JA, Ulrich ED, Adamson CR, Zukoski CF. Identification of prolactin-like proteins synthesized by normal murine lymphocytes. Endocrinology. 1990 Nov;127(5):2601–2603. [PubMed] [Google Scholar]
  • Kiefer KA, Malarkey WB. Size heterogeneity of human prolactin in CSF and serum: experimental conditions that alter gel filtration patterns. J Clin Endocrinol Metab. 1978 Jan;46(1):119–124. [PubMed] [Google Scholar]

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