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Deoxyribonucleate Binding and Transformation in Rhizobium japonicum

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PMC

US National Library of Medicine
National Institutes of Health J Bacteriol. 1972 Aug; 111(2): 356–360. PMCID: PMC251290 PMID: 4538250

Deoxyribonucleate Binding and Transformation in Rhizobium japonicum

J. L. Raina and V. V. Modi Author information Copyright and License information Disclaimer Department of Microbiology, M. S. University of Baroda, Baroda, India Copyright notice This article has been cited by other articles in PMC.

Abstract

Rhizobium japonicum, capable of binding high-molecular-weight donor 32P-labeled deoxyribonucleic acid (DNA) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. Eight to 10% of the homologous native 32P-labeled input DNA was bound irreversibly in a temperature-dependent manner. Homologous denatured 32P-labeled DNA was incapable of binding to the recipient under similar conditions. CsCl density gradient banding of the donor and recipient DNA indicated homology. The low frequency of transformation could be due to one or more steps that occur between DNA uptake and integration.

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Selected References

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