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Western Blotting - PubMed

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Actions . 2006 Apr;38(4):283-93. doi: 10.1016/j.ymeth.2005.11.007.

Western Blotting

Biji T Kurien  1 R Hal Scofield Affiliations

Affiliation

  • 1 Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA. [email protected]
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Western Blotting

Biji T Kurien et al. Methods. 2006 Apr. Actions . 2006 Apr;38(4):283-93. doi: 10.1016/j.ymeth.2005.11.007.

Authors

Biji T Kurien  1 R Hal Scofield

Affiliation

  • 1 Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA. [email protected]
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Abstract

Western blotting (protein blotting or immunoblotting) is a powerful and important procedure for the immunodetection of proteins post-electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer. This review describes the various procedures that have been used to transfer proteins from a gel to a membrane based on the principles of simple diffusion, vacuum-assisted solvent flow and electrophoretic elution. Finally, a brief description of methods generally used to detect antigens on blots is also described.

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